Positive Gram Coconuts

Positive Catalase, has formation of bubble on of plates, in case that I oppose is catalase negativa.3.2 Test of coagulasePosteriormente the Catalase became the test of Coagulase in all the colonies that had presented resulted positive in the test of catalase. This test has for objective to verify or not it presence of the Coagulase enzyme. This active enzyme plasma coagulation factors harvested with anticoagulating Citrate. The Staphylococcus aureus possesss this enzyme. After the preparation of a suspension from the colonies of positive Positive Gram Coconuts catalase, was incubou in microbiological greenhouse, aliquot of 200 l together with plasma barren of rabbit harvested with citrate for 24h 36C, in barren glass pipe. Ribeiro; To sound (2002), affirm that in case of formation of cogulo the bacterium in question is of the Staphylococcus species aureus.4 Test of NovobiocinSimultaneamente to the test of the Coagulase became fullfilled the test of the Novobiocina for all the coconuts positive gram-positive catalase. In case of negative Coagulase this test serves to differentiate the main not producing species of Estafilococos of coagulase.

This test was carried through becoming it sowing of the bacterium in question on a plate of Agar blood, assists with it of one swab barren. On this sowing a record of Novobiocina was placed and the plate was incubada in microbiological greenhouse for 24h to 36C. After this period of time was become fullfilled measured it of the inhibition halo, where this occurred. Levi (2004) affirms that the resistant samples show to zones of inhibition of 6 12 mm, and most sensible they present halos of 16 mm more or. Cepas of epidermidis Staphylococcus saprophyticus is resistant and of Staphylococcus they are sensveis.5 Identification of EnterobactriasAs negative Gram bacteria that had grown in the MacConkey agar, had been submitted the tests stops identification using itself the Kit for Enterobatrias of company NEWPROV.